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Each 24 h 20-40 mg of PtO2 was included plus the development in the response monitored by 31P NMR spectroscopy. Just after removing from the catalyst by filtration along with the solvent by evaporation, the solid residue was washed with ethyl ether to get N

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-trifluoroacetyl substituted substrate is hydrolyzed 26 times quicker compared to organic substrate nevertheless the N

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It could be argued, nevertheless, that if NagA ended up constrained by item release through the active website, then the dissociation of trifluoroacetate could be inherently quicker than the release of acetate. This distinct scenario was dealt with with the utilization of alterations in solvent viscosity to systematically change the speed constants with the association and dissociation of goods and substrates with NagA (19, 20).

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